Easy Science Experiment: Grow Sulfur Loving Bacteria With Your Kids!

Last Updated on February 20, 2021 by Diane Hoffmaster

Looking for an easy science experiment for kids to teach them to love STEM classes?  Learn how to build a Winogradsky column and grow sulfur loving bacteria with your children!  

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collage of pond water and DIY winogradsky column showing orange bacterial growth

Teaching Kids to Love STEM Subjects

My husband and I both have degrees in microbiology.  While I haven’t done much with my degree since having kids, I feel it is important to share our love of science with our children.  Personally, I don’t have a lot of faith in the ability of the public school system to prepare my kids for college.

Unfortunately, I can’t afford private school and would probably strangle my children if forced to home school them so it is off to public school they go. We frequently do easy science experiments at home and have an extensive collection of books to help us come up with new ideas.  Here is a very easy science experiment that  you can do at home. 

You need only a few easy to find ingredients and supplies but you will have to have PATIENCE to see the results.  We are talking a few months worth of patience!  But, the results really are rather astounding! 

To build our Winogradsky Column, we followed the directions from The Science Education Resource Center.  This is EASY, you and your kids can do this together and have fun while learning something totally cool!

The Science Behind This STEM Experiment

For this experiment, you are building a  Winogradsky column which is a simple device for culturing a large variety of microorganisms.  It was invented by Sergei Winogradsky and contains  pond mud and water mixed with a carbon source such as newspaper, blackened marshmallows or egg-shells. 

You will also need  a sulfur source such as gypsum or egg-yolk.  Incubating the column in indirect sunlight for months results in a gradient of different oxygen levels, nutrient types and sulfur levels. 

You can read more about the science behind this experiment at the link above.  It will explain all about the different gradients and where certain bacteria prefer to live.  Here is a how to video that may help you put it together.  Detailed directions are included below.

NOTE:  The column is a rough mixture of ingredients  so exact measurements are not critical.


How to build a Winogradsky Column


1.  A tall glass or plastic container (we used a large glass peanut jar that we washed and dried)

2. Small shovel for scooping mud

3.  Small bucket to carry mud and pond water in

4.  Carbon source:  Vegetable materials such as shredded hay, grass clippings, shredded newspaper, sawdust, corn starch, oatmeal, will work fine, use your imagination. (we used oatmeal)

5.  Sulfur source:  elemental sulfur, calcium sulfate, magnesium sulfate, raw or hard boiled eggs, or cheese.  (we used a raw egg!)

6.  Stick for stirring


1.  Head out to your closest pond with a bucket and shovel.  Collect a small amount of mud and pond water.  You only need enough to fill your container so don’t worry about getting a whole bunch.  Try not to get a lot of sticks or rocks.  You want relatively ‘clean’ mud!

2. Back in the kitchen, grab a small bowl and mix a couple scoops of pond mud with the carbon and sulfur sources.  We used about 2 – 3 cups (maybe?) of mud with one raw egg and about 1/2 cup of oatmeal.  Mix the concoction thoroughly.

3.  Add some pond water to your incubating container and add the soil mix from above a little at a time, using a stick to pack the material in the bottom of the column.  Try to avoid air bubbles as much as possible.

4.  Add water and soil mixture until the column is about three fourths full.

5.  Leave some air space above the surface of the water and cover the column with plastic wrap or a very loose lid.

6.  Place in a sunny location but NOT in direct sunlight.  (ie in a sunny room but not on the windowsill) and WAIT.


Our Winogradsky Column Results

Here are photos of our experiment.  The first photo (on the left) was taken in September of 2011 and the photo on the right was taken in February of 2012.  Incubation time was about 4 months but we started seeing small changes after about 8 weeks.

Each portion of the column allows for the growth of very specific types of organisms.  See photos and descriptions of each zone HERE but take a close look at my picture.  See that RED line in the photo on the right? 

That is a very high concentration of purple sulfur bacteria  They love to grow in areas that are low in oxygen but high in sulfur.  Just like the bottom of your Winogradsky Column!  This organism is present naturally in the mud at the bottom of the pond, you are just increasing the concentration enough so you can see it clearly.

 I hope you learned something interesting and are inspired to go out and explore the world of science with your children!  If you decide to try this easy science experiment out for yourself, stop back by and let me know how it goes!


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  1. I bet it would smell to high heavens if you opened it up! Neat experiment!
  2. that is so gross, it's fab, lol
  3. This is a fun way to teach your kids about microbiology.
  4. That is pretty neat and pretty gross too!
  5. yes, science is messy and icky and sometimes stinky but also a whole bunch of fun!
  6. Now that is cool! I'm sharing this in my homeschool group.
  7. Cedrick Grageda says
    Thanks for the details on Easy Science Experiment: Grow Sulfur Loving Bacteria With Your Kid!, it will likely be truly beneficial.
  8. This is such a cool experiment!
  9. This is something that my kids would love... Also, stopping by from Pitch it to me. Minta
  10. Ya know, that was a freshman year microbiology lab experiment for me. I was just remembering it and thinking it would make a cool visual experiment for demonstrating soil bacteria to people. Good one.
    • Glad to have brought back fond memories! I was a microbiology major in college but we didn't do this particular experiment. Kids had fun with it at home though!

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